Special Lecture: Mechanical Characterization of Cells and Development of Bio-MEMS Devices for Cell Culture Study

IIT Hyderabad will organize a special lecture by Dr. Toshiro Ohashi, Hokkaido University, with the following detail.

30th October 2017 (Mon)
VenueRoom 118 (Academic Building A)
SpeakerDr. Toshiro Ohashi, Professor, Faculty of Engineering, Hokkaido University
Title"Mechanical Characterization of Cells and Development of Bio-MEMS Devices for Cell Culture Study"

Special Lecture Abstract

A lot of efforts have been made to study the effects of mechanical stimuli on cell remodeling, however, little is still known of how mechanical forces are transmitted through cells to activate intracellular signalling cascades leading to alterations in cell functions. To further address this issue, it would be required to know intracellular mechanical environment including mechanical properties of subcellular structural components such as actin filaments, nucleus and so forth. The aim of this talk is to present recent findings related to cell biomechanics, introducing mechanical tests of cell body as well as intracellular organelles. Our research work on applications of bio-MEMS technologies to cell biomechanics is also presented.

CV of Dr. Toshiro Ohashi

Toshiro Ohashi, Ph.D., Professor
Faculty of Engineering, Hokkaido University

2000 Ph.D., Graduate School of Engineering, Tohoku University, Japan

1994 M.S., Graduate School of Engineering, Tsukuba University, Japan
1991 B.S., School of Mechanical Engineering, Tsukuba University, Japan

Positions and Honors
Academic positions:

2014 Visiting Professor, Department of Biomedical Engineering, Universiti Teknologi Malaysia, Malaysia
2012 Visiting Lecturer, Institute for Frontier Medical Sciences, Kyoto University, Japan (to 2013)
2010 Visiting Professor, Institute for Materials Chemistry and Engineering, Kyushu University, Japan
2009 Professor, Mechanical Engineering, Hokkaido University, Japan (to current)
2008 Visiting Scientist, Royal Institute of Technology, Sweden (to 2008)
2004 Visiting Scientist, Queen Mary University of London, UK (to 2005)
2002 Associate Professor, Mechanical Engineering, Tohoku University, Japan
1994 Research Associate, Mechanical Engineering, Tohoku University, Japan
2013 Editor-in-Chief, Journal of Biorheology (to current)
2010 Editorial Advisory Board, Bio-Medical Materials and Engineering (to current)
2010 Associate Editor, Journal of Biomechanical Science and Engineering (JBSE) (to current)
2014 Poster Award at the 4th Japan-Switzerland Workshop on Biomechanics (Shima, Mie, Japan, Sept 1 -4 , 2014)
2013 JSME Medal for Outstanding Paper in 2012, JSME
2012 JBSE Papers of the Year 2010
2009 JSME Medal for Outstanding Paper in 2008, JSME
2008 JSME Medal for Outstanding Paper in 2007, JSME
2004 Young Investigators Award of JSMBE
2004 The Seguchi Prize (12th) in Bioengineering, Bioengineering Division, JSME
2002 Outstanding Paper Award at International Congress on Biological and Medical Engineering (Singapore, Dec 4-7, 2002)
2002 Young Investigators Award of JSMBE
2001 Award for Best Poster at 2001 Summer Bioengineering Conference (Snowbird, Utah, USA, June 27- July 1, 2001)
2000 Outstanding paper award at the 10th International Conference on Biomedical Engineering (Singapore, 6-9 Dec, 2000)
2000 JSME Medal for Outstanding Paper in 1999, JSME
Academic societies:
JSME (Japan Society of Mechanical Engineers), JSMBE (Japanese Society for Medical and Biological Engineering),
Japanese Society of Biorheology, ASME (American Society of Mechanical Engineers), JSEM (Japanese Society for Experimental Mechanics)

Research Interests

Mechanical Engineering, Cell Biomechanics, Vascular Wall Biomechanics, Bio-MEMS
Cell/Tissue Biomechanics (understanding of cell remodeling process in response to external mechanical stimuli / mechanical characterization of cells and soft tissues in health and disease) Bio-MEMS (development of PDMS-made engineering matrix for cell analysis)

Publications (Recent 10 Papers out of 73 papers in total)
1. T. Ohashi, Y. Sugaya, N. Sakamoto, M. Sato, Relative contribution of physiological hydrostatic pressure and fluid shear stress to endothelial monolayer integrity, Biomedical Engineering Letters, Vol. 6, Issue 1, pp 31–38, 2016.
2. E. Maeda, T. Ohashi, Mechano-regulation of gap junction communications between tendon cells is dependent on the magnitude of tensile strain, Biochemical and Biophysical Research Communications, Vol. 465, No. 2, pp. 281-286, 2015.
3. S. Chen, A. Morita, I. Sukmana, E. Maeda, T. Ohashi, Development of fibrin gel-microgroove model for microvascularization by endothelial cells, Journal of Biorheology, Vol. 29. Issue 1, pp. 19-23, 2015.
4. S. Danworaphong, M. Tomoda, Y. Matsumoto, O. Matsuda, T. Ohashi, H. Watanabe, M. Nagayama, K. Gohara, P.H. Otsuka, O.B. Wright, Three-dimensional imaging of biological cells with picosecond ultrasonics, Applied Physics Letters, Vol. 106, pp. 163701-1- 163701-5, 2015.
5. E. Maeda, M. Sugimoto, Y. Kosato, C.T. Poon, B. Pingguan-Murphy, T. Ohashi, Tenocyte anabolic and catabolic response to elevation in intracellular tension, Journal of Biorheology, Vol. 28. Issue 1, pp. 16–20, 2014.
6. E. Maeda, T. Tsutsumi, N. Kitamura, T. Kurokawa, J.P. Gongc K. Yasuda, T. Ohashi, Significant increase in the Young’s modulus of ATDC5 cells during chondrogenic differentiation induced by PAMPS/PDMAAm double-network gel: Comparison with induction by insulin, Journal of Biomechanics, Vol. 47, No. 13, 3408-3414, 2014.
7. E. Weibull, S. Matsui, H. Andersson-Svahn, T. Ohashi: A microfluidic device towards shear stress analysis of clonal expanded endothelial cells, Journal of Biomechanical Science and Engineering, Vol. 9, No. 1, pp. JBSE0006, 2014.
8. E. Weibull, S. Matsui, M. Sakai, H. Andersson-Svahn, T. Ohashi, Microfluidic device for generating a stepwise concentration gradient on a microwell slide for cell analysis, Biomicrofluidics, Vol.7, Issue 6, 064115-1- 064115-12, 2013.
9. E. Maeda, Y. Hagiwara, J. H-C. Wang, T. Ohashi: A New Experimental System for Simultaneous Application of Cyclic Tensile Strain and Fluid Shear Stress to Tenocytes in Vitro. Biomedical Microdevices, Vol. 15, pp. 1067-1075, 2013.
10. E. Maeda, M. Sugimoto, T. Ohashi: Cytoskeletal Tension Modulates MMP-1 Gene Expression from Tenocytes on Micropillar Substrates. Journal of Biomechanics, Vol. 46, pp. 991-997, 2013.